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Aliquots from the reverse-transcribed samples were used for the detection of specific mRNA transcripts of different genes of interest by RTqPCR using commercially available mouse cDNA sequence-specific PCR primers and Taqman probes

Mutant #14 exhibited the highest improvement, with a 2.3-fold increase in intracellular glutathione content and a 3.2-fold increase in concentration relative to the wild-type strain (Fig

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